Research Topics

공지 학부연구원 및 대학원생 모집 공고 관리자 2021-11-17 287

3D Bioprinted Organ-on-a-chip

Currently confidential

Stem Cell Tissue Engineering and Bioprocessing

To dates, as stem cells are considered a promising cell source for tissue engineering and regenerative medicine, many culture strategies have been extensively studied to generate in vitro stem cell-based three-dimensional (3D) tissue constructs. Previously, we have reported 3D-osteogenesis of stem cells could be well induced by an integrated 3D-bioprocess composed of hydrogel encapsulation and a fed-batch rotating wall vessel (RWV) bioreactor. However, such a fed-batch based 3D culture system appeared to be limited in a real-time control of nutrients, metabolites, oxygen, and other molecules transport, which hinders to support optimal culture environments for stem cells’ biological behaviors in proliferation, differentiation, and tissue formation. Therefore, in our lab, we designed a novel, automated perfusion RWV bioreactor for continuous supply of nutrients/oxygen and removal of metabolic wastes during the whole culture period to establish optimal culture condition for stem cell-based 3D tissue formation. Our new bioreactor demonstrated highly reproducible ...

Therapeutic EV Bioprocessing

At present, various clinical trials using mesenchymal stem cells (MSCs) have taken place with increasing success rates. Given that their clinical efficacy is mainly attributed to paracrine effects, recent studies have drawn much attention to extracellular vesicles (EVs) secreted from MSCs. These bilipid-layered and nano-sized vesicles retaining medicinal potency equivalent to that of MSCs could reach a safe and effective cell-free treatment while circumventing current limitations of stem cell infusion therapies. 1) Technical challenges in obtaining sufficient amounts of EVs have limited further progress in clinical applications. Of the multiple efforts to reinforce the therapeutic capacity of MSCs, few studies have reportedly examined the scale-up of MSC-derived EV production. In our lab, we successfully amplified EV secretion from MSCs compared to the conventional culture method using a simple and efficient 3D-bioprocessing method. Size-controlled human MSC spheroids were homogeneously formed on a large sc ...